You have discovered several mutant strains of E.co. Each mutant exhibits a defec

Question

You have discovered several mutant strains of E.co. Each mutant exhibits a defect in DNA replication. To assess the defect in each strain, you isolate and analyze the double-stranded nucleic acids. From the results below,list the replication enzyme or protein you believe isn't functioning in each strain, and give a very brief explanation Be as specific as possible (in other words, if an enzyme has multiple activities, specify which activity is affected). For example, if a strain's nucleic acid consisted of one very long DNA molecule on one strand and a molecule of DNA on the other strand that contained numerous mismatched bases, you might conclude that the 3 to 5' exonuclease activity of DNA polymerase Ill wasn't functioning, because proofreading apparently hadn't occurred during replication a) Strain 1: the nucleic acid consists of a very long DNA molecule on one strand, and numerous short RNA molecules on the other strand bi Strain 2: the nucleic acid consists of two very long DNA molecules, each comprising one strand of the double stranded nucleic acid c Strain 3: the nucleic acld consists of a very long DNA molecule on one strand and numerous short stretches of nucleic acid consisting of RNA joined to DNA on the other strand d) Strain 4: The nucleic acid consists of a very long DNA molecule on one strand and numerous short molecules of DNA only on the other strand Strain 5: The nucleic acid consists of very long molecules of single-stranded ONA only Paragrapho

Explanation / Answer

1. DNA polymerase 3 is not functional because RNA primer are attached yet no synthesis starts.

2. Helicase is non functional because it unwind and form a fork for Polymerase function.

3. DNA pol 1 is not functional because it cleaves RNA primers and fill the gap.

4. DNA ligas which join okazaki fragments is not functional.

5. Primase is non functional which start replication by insertion of a small RNA sequence on single strand to start DNA synthesis.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.