Ex. 2A: Vitamin C and 1.0% Nalco, . Change in pH of Urine over Time. Both Vitami

Question

Ex. 2A: Vitamin C and 1.0% Nalco, . Change in pH of Urine over Time. Both Vitamin C and NaHCO, can affect your body's pH homeostasis. If extracellular is too acidic, the kidneys help regulate pH through excretion of urine that contans H'ions and reabsorption of bicarbonate ion (HCOp-) which act as a buffer. If fluid is too alkaline, the kidneys can remove HCOs and conserve H". To test how these two substances, affect plH, a student test subject drank 500ml of water with 1000 ng of vitamin C and another drank 500mL of 1.0% NaHCO. Their urine volume rate, color, specific gravity, and pH were recorded in 20-minute intervals for 100 minutes total (Table 2A.1 and 2A.2 below), Generate a hypothesis and then graph the changes in urine pH over time for Vitamin C and i% NaHCO. Graph both sets of data on the same graph. Make a line or scatterplot graph of pH vs. time. Discuss your hypotheses and results on the worksheet. Table 2A.1: Vitamin C Volume Rate Color dark yellow Time (min) 1.010 160 1.005 Table 2.1.2: 1.0% NaHCO Volume Rate pH 75 40 80 clear 15 Ex. 2A: Vitamin C and 1.0% NaHCO-Change in pH of Urine over Time from p.2) 7a State your hypothesis for vitamin C and 96 Nalah Explain your reasoning based on what you know about rawy ertime fr Vitamin C and 1% Nalco. You will have hes. Label t Time and the Y-axis 7b)Graph changes nun e pH pH" (include units) xaus Change in pH of Urine over Time Vitamin C NaHCO3 020406080100 120 Time (min) ? Re)Disussshe resultsofthis ex

Explanation / Answer

7(a) When sodium bicarbonate was administered the urine was highly alkaline due to significant decrease in ascorbic acid content and this tend to increase the pH that can equally understood if consider the graph shown in 7(b) while, simply administration of vitamin c i.e. a reducing agent which donates its electron and proton and become oxidized itself. However, it is tough to clinically defined the experiment but just for hypothesis purpose it showed significant decrease in pH of the urine with the increase in period of interval as showed in the graph.

&(c) The result interpretation is quiet clear that consumption of vitamin c (i.e.a source of ascorbic acid) causes fall in ph of the urine with the incearse in time interval while simply consumption of 1% sodium bicarbonate shoot the ph of the urine with effect of time.

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