1. In the “Effects of NF-kB siRNA on the expressions of NF-kB and NF-kB p65 in l

Question

1. In the “Effects of NF-kB siRNA on the expressions of NF-kB and NF-kB p65 in lung tissues of LPS-induced ALI rats” section in the results section the authors wrote “At 1 h after LPS exposure, the level of NF-kB mRNA transcription in LPS + siRNA group was significantly reduced (0.297) compared with that in LPS + scramble group (0.927). Similarly, at 8 h after LPS exposure, NF-kB transcript levels in LPS + siRNA group was significantly reduced (0.607) (Fig. 2a).” Based on this, what is siRNA doing? (1 point)

2. How do you know that your answer to question 1 is correct? (1 point)

3. In the “Effects of NF-kB siRNA on the rectal temperature, white blood cell counts, and lung wet/dry weight ratio of LPS-induced ALI rats” section of the results section the authors wrote “Compared with control group, the lung wet/dry weight ratio was obviously enhanced after LPS administration at each time point. Rats with NF-kB siRNA installation has a reduced lung wet/dry weight ratio.” What does a reduced lung wet/dry weight ratio indicate? (1 point)

4. Why would saline + DEPC have the lowest wet/dry ratio? (1 point)

Explanation / Answer

1. siRNA reduces transcript levels.

2. Because siRNA was significantly reduced after LPS exposure.

3. Reduced lung wet/dry weight ratio indicates the reduction in total proteins and nuclear cell count.

4. This is because hydrogen rich saline protect against lung.

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