Labeled pyruvate containing 14C ( 200 mCi/gm at ) C-3 ( the methyl carbon), 13 C
ID: 35146 • Letter: L
Question
Labeled pyruvate containing 14C ( 200 mCi/gm at ) C-3 ( the methyl carbon), 13 C( 90% enriched) at C-2 and 18 O ( 80%enriched) at C-2m was added to a pigeon breast muscle preparation, in order to investigate the citric acid cycle.
What will the labeling patern be ( both postion and extent of labeling ) in the isolated a- ketogluterate and in oxaloacetate ( Assume label enters onlin in the first turn ( ie pulse of labeled pyruvate is followed by a chase of unlabeled pyruvate.))
-- A ketoglutarate
18O
--- Oxaloacetate
18O
14 C
13 C
1st cycle 2nd cycle 3rd cycle-- A ketoglutarate
18O
14 C 13 C--- Oxaloacetate
18O
14 C
13 C
Explanation / Answer
1st
2nd
3rd
18 O
It will be incorporated at C1 position as
1st
2nd
3rd
- Ketoglutarate
18 O
It will be incorporated at C1 position as
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