And Question #2 Rhodospirillun Rubrum instead of tooth and gum scraping. Lab Sec

Question

And Question #2 Rhodospirillun Rubrum instead of tooth and gum scraping. Lab Section 40871 Name: Shantelle Toedg LABORATORY REPORT Negative Staining Date: PURPOSE EXPECTED RESULTS 1. Sketch a negatively stained rod: 2. Which organism used in this exercise is a rod? Pacilus wiohiis RESULTS Sketch a few bacteria (viewed with the oil immersion objective lens). Specimen: Bacillus subtilis Staphylococcus epidermidisTeethr and gum scraptng Total magnification: Morphology and arrangement CONCLUSIONS l. Which cell is a rod? bacill tilis How does its appearance differ from that of the rod you tained with methylene blue simple,diret stain? Thcy look iwew shadout ckax. 53

Explanation / Answer

Staphylococcus epidermidis & Rhodospirillum rubrum are observed in tooth & gum scraping.

The main purpose of negative staining is to study the morphological shape, size and arrangement of bacterial cells that is difficult to stain, eg., Spirilla. It can also be used to stain cells that are too delicate to be heat fixed.

Negative stainging requires a acidic dye such as India ink or Nigrosin.

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