1. In this experiment, DNS is converted to ANS. The optical density of ANS in so
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Question
1. In this experiment, DNS is converted to ANS. The optical density of ANS in solution is used as an indicator of sucrase activity. Why?
more bile acids would be lost to feces after a standard meal compared to the fiber-rich meal.
there would be no difference in loss of bile acids to the feces after the standard meal compared to the fiber-rich meal.
less bile acids would be lost to feces after the standard meal compared to the fiber-rich meal.
3. During the experiment on adsorption of bile acids to food,
less bile acids adsorbed to the contents from the standard meal compared to the fiber-rich meal.
there was no difference in adsorption of bile acids in the standard meal compared to the fiber-rich meal.
more bile acids adsorbed to the contents from the standard meal compared to the fiber-rich meal.
the optical density of the solution from the standard meal was higher than optical density of the solution from the fiber-rich meal.
the optical density of the solution from the standard meal was lower than optical density of the solution from the fiber-rich meal.
there was no difference between the optical density of the solution from the standard meal and the optical density of the solution from the fiber-rich meal.
5. A friend asks your advice about adding fiber to her diet. She wondered whether adding fiber would cause her to eat less (because she feels fuller) and whether fiber would help prevent constipation (because it decreases transit time). Based on the results of this experiment, you recommend that she do
add only soluble fiber to her diet because insoluble fiber had no effect on either activity.
add both types of fiber to her diet because each affected one activity more than the other.
add only insoluble fiber to her diet because soluble fiber had no effect on either activity.
not add fiber because fiber had no effect on either activity .
Amount of DNS that is converted to ANS is proportional to the amount of glucose and fructose that is formedExplanation / Answer
1. answer is amount of DNS converted in to ANS indicates how much is denatured
Sucrose solutions - with and without active sucrase - are hydrolyzed during a 20-minute incubation. Following incubation, DNS (dinitrosalicylic acid) is added. The pH of the DNS solution denatures sucrase and stops the reaction, thus fixing the concentration of the reaction products.
After heating the solutions to 90°C (194°F), DNS will convert to ANS (3-amino-5 nitrosalicylic acid) if a monosaccharide - like glucose or fructose - is present. The amount of ANS, a reddish-brown colored chemical, is determined using a spectrophotometer, an instrument that measures the amount of light that is transmitted through a colored solution. The more ANS in the solution, the greater the opticaldensity will be. The amount of ANS produced is analogous to the amount of product produced from the enzyme reaction.
2. fiber increases the amount of bile lost in the feces
less bile acids would be lost to feces after the standard meal compared to the fiber-rich meal.
3. more bile acids adsorbed to the contents from the standard meal compared to the fiber-rich meal.
4. standard meal with bile acid had the greatest optical dfensity
the optical density of the solution from the standard meal was higher than optical density of the solution from the fiber-rich meal.
5.add both types of fiber to her diet because each affected one activity more than the other.
Soluble fiber turns into a gel when mixed with water in your gastrointestinal tract. Because soluble fiber delays stomach emptying, it makes you feel fuller and thus makes you less prone to overeating. This obviously helps to control a person's weight.
insoluble fiber. This is a type of fiber that helps to add bulk to a stool, pass stool more quickly, and thereby prevent things such as hemorrhoids, constipation, and more
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