Explain how the following mutations would affect the transcription of the yeast

Question

Explain how the following mutations would affect the transcription of the yeast GAL1 gene.

A deletion within the GAL4 gene that removes the region encoding amino acids 1 to 100.

A deletion within the GAL4 gene that removes the region encoding amino acids 1 to 100.

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Part B

A deletion of the entire GAL3 gene.

A deletion of the entire GAL3 gene.

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Part C

A mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p.

A mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p.

A deletion within the GAL4 gene that removes amino acids 1-100 would remove the DNA-binding section but allow transcriptional activation. A deletion within the GAL4 gene that removes amino acids 1-100 would reduce transcription of the GAL1 gene. A deletion within the GAL4 gene that removes amino acids 1-100 would remove the DNA-binding section and not allow transcriptional activation.

Explanation / Answer

Part A

A deletion within the GAL4 gene that removes amino acids 1-100 would remove the DNA-binding section and not allow transcriptional activation.

Part B

Without the product of the GAL3 gene, there would be no disruption of the Gal4p/Gal80p complex and therefore no transcription of the GAL1 gene.

Part C

If the GAL80 gene product can

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