5. You put a solution of salivary amylase into a tube. You add trypsin to the tu

Question

5. You put a solution of salivary amylase into a tube. You add trypsin to the tube. One hour later, you add starch to the tube. What will be present in your tube after another hour has passed? 6. You have a five test tubes. These tubes (#1-45) contain solutions of substances as me specified below. Describe what would be in each of these tubes after any enzy reaction(s) go to completion: Tube #1 : lactose, lactase, maltose, maltase, sucrose be #2: sucrose, galactose, starch, amylase, dextrinase, and glucoamylase Tube #3: triglycerides, bile salts, pancreatic lipase, sucrose, sucrase Tube #4: pepsinogen, myosin, HCI Tube # 5: pepsinogen, myosin

Explanation / Answer

5. trypsin cleaves at carboxyl side of lysine and arginine of any any protein. When trypsin is added to a solution of salivary amylase, it cleaves the amylase at lysine and arginine, so that amylase is deactivated. Usually amylase hydrolyzes starch into disaccharides and trisaccharides. However, since the amylase is degraded now, it cannot act on starch or hydrolyze starch.

6.

Tube 1: lactase acts on lactose to give glucose and galactose. Maltase hydrolyzes maltose to glucose. Sucrose will not fit into the active site of either lactase or maltase. At the end of reaction, tube 1 consists of glucose, galactose and sucrose.

Tube 2: amylase acts on starch to produce maltose and maltotriose.

Dextrinase degrades amylopectin component of starch to produce amylose.

Glucoamylase removes glucose units from starch.

End products would be sucrose, galactose and glucose.

Tube 3:

Lipase converts triglycerides into monoglycerides and two fatty acids.

Sucrose hydrolyzes sucrose to fructose and glucose.

End products would be monoglycerides, fatty acids, fructose and glucose.

Tube 4:

Pepsinogen is activated by HCl to pepsin. Pepsin cleaves proteins. In this tube, pepsin cleaves myosin.

Tube 5;

Pepsinogen is an inactive enzyme. It cannot act on myosin.

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