The antibiotic puromycin blocks genetic translation. The antibiotic actinomycinD

Question

The antibiotic puromycin blocks genetic translation. The antibiotic actinomycinD blocks genetic transcription. These can be used to experimentally determine the mechanism of action for physiologic molecules. The effect of a hormone is immediately blocked by puromycin, but was not blocked by actinomycinD. From this you can conclude that the hormone might a. regulate protein synthesis by ribosomes from existing mRNA b. regulate protein degradation of active proteins by the proteosome. c. bind DNA as a regulator of mRNA synthesis from DNA template d. be needed for nucleic acid synthesis e. be needed for amino acid synthesis Alternative splicing of exons results In: a. ribosomal assembly in the nucleolus b. the production of siRNA, miRNA. and RNA silencing c. the production of different mRNA molecules from a common precursor RNA d. posttranslational modifications of proteins e. the existence of a genome that is larger than the proteome

Explanation / Answer

question 3: there can be two answers as the hormone is blocked by puromycin the genetic translation is blocked and for this precise answer can be A or little ambiguous answer can be E.

Question 4 : The answer is C. The splicing results in multiple proteins from a single gene.

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