2. We wish to map the pro gene quickly to the genes arg leu bio in the above exp
ID: 34406 • Letter: 2
Question
2. We wish to map the pro gene quickly to the genes arg leu bio in the above experiment. So we isolate
DNA from a bacteria that is wild type for the genes arg leu pro, shear it in a kitchen blender we bought at
WalMart, mix it into a culture of bacteria that is mutant for the genes arg leu pro. (We are not bothering
with bio right away.) We then plate on various minimal media. These are the results:
Media with arginine, leucine, and proline 10000 colonies/ml
Media with arginine 10 colonies /ml
Media with proline 100 colonies /ml
Media with leucine 50 colonies /ml
a) What is this experiment called?
b) Considering the combinations of genotypes we are using, which will grow on media with proline?
c) What is the order of the three genes.
d) Now, bio, we didn
Explanation / Answer
a)
The experiment is called genetic mapping or recombination mapping.
b)
The bacterium with genotype pro- grows on medium with proline.
c)
The order og genes is arginine, leucine and proline (number of colonies of individual amino acid/ number of colonies grown on all the three amino acids or 10/10000, 100/10000 and 50/10000 = 0.001, 0.01 and 0.005 respectively).
d)
The gene bio can be placed between leucine and proline.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.