The predominate form of an important buffer in our blood (pH 7.4) is a H2CO CO d

Question

The predominate form of an important buffer in our blood (pH 7.4) is a H2CO CO d OH e H20 To detect peptides eluting from a reversed-phase column, Prof. Dimwit monitors the absorbance at 280 nm instead of 214 nm. Among the few peptides that he detects are those containing at least one a His b Trp c Ser e Pro After cleavage of the polypeptide PSEGK DDNSR FWILK GAPEV with trypsin, 4 peptides were isolated by reversed-phase HPLC. The peptide that eluted last from the column was subjected to Edman sequencing. The first cycle yielded PTc- a Pro b Val c Asp d Gly Consider the reaction AB, where G° =-774 kJ/mol. If the steady-state concentration of A is maintained at 1 mM in a cell at 37°C, what condition is required for the reaction to proceed in the forward direction (A B)? a B] 20 mM (2 c B]0.05 mM e This reaction will never go forward when [A]- 1 mM Here is a Hill plot of O2 binding to hemoglobin (Hb). 2.0 1.0- 2.0 3.0 1.5 1.0 0.5 0.0 0.5 10 .5 2.0 253.0 3.5 log(PO2) a. Which line (A, B, C or D) corresponds to the behavior of the Hb if all the subunits were stuck in the T-state (low affinity for O2) at all values of PO2? b. Which line (A, B, C or D) is used to estimate the Hill coefficient? c. The Hill coefficient for a 4-subunit protein can never exceed what value?-AD

Explanation / Answer

HCO3

Recognize the acids produced by the body during metabolism and be able to state the predominant form at pH = 7.4

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