4. Hydropathy index vs. Rr. Based on your data for the A, v, L and P standards,

Question

4. Hydropathy index vs. Rr. Based on your data for the A, v, L and P standards, does their hydropathy index correlate with their observed Rt values? Explain. A B drophobl C for most rart 42. 1.3 P. 31 ,si 1. RAC almost same in boh 5. Chromatography- Mechanism of Retention. Predict the elution order of the following amino acids under conditions for either HILIC or reverse-phase chromatography at pH 2.S. Use a "1" for the component eluting first. Hint. You may want to note the hydropathy index values of Table 1 (above) Amino Predicted Elution Order Acid Reverse-Phase HILIC

Explanation / Answer

AMINOACIDS

PREDICTED ELUTION ORDER

HILIC

REVERSE PHASE

R

6

7

G

5

2

S

4

1

V

2

4

T

8

6

A

3

3

M

1

5

F

7

8

AMINOACIDS

PREDICTED ELUTION ORDER

HILIC

REVERSE PHASE

R

6

7

G

5

2

S

4

1

V

2

4

T

8

6

A

3

3

M

1

5

F

7

8

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