Recent work published in Cell (2014) by Pagliuca et al, described a technique to

Question

Recent work published in Cell (2014) by Pagliuca et al, described a technique to generate many millions of pancreatic beta cells from human pluripotent cells in vitro.

a) Using this approach, why is it important to generate homogeneous populations of beta cells?

b) What techniques can be used to test for and increase homogeneity in mixed cell populations?

c) The beta cells were expanded as cellular aggregates grown in suspension culture using a bioreactor. Which key features of the growth conditions would need to be optimised to maximize cell viability?

d) How would you test if the beta cells functioned in vivo?

Explanation / Answer

a) The beta cells are derived from pluripotent stem cells that have teratoma (tumour)

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