Explain in words the proper steps to use in the following \"specific transfer\"

Question

Explain in words the proper steps to use in the following "specific transfer" Staphylococcus epidermidis from broth to a slant.
Explain why it is necessary to do different dilutions with the spread plate method. Explain in words the proper steps to use in the following "specific transfer" Staphylococcus epidermidis from broth to a slant.
Explain why it is necessary to do different dilutions with the spread plate method.
Explain why it is necessary to do different dilutions with the spread plate method.

Explanation / Answer

The proper steps to use in specific transfer of Staphylococcus epidermidis from broth to a slant.

1. Sterilize the loop by exposing it to the flame in a laminar flow hood. Let the loop cool on its own.

2. Sterilize the mouth of the tube- open the tube in a laminar flow hood. Sterilize the mouth of the tube by briefly exposing to the flame.

3.. Take sterile loop and dip it into the broth of the Staphylococcus epidermidis bacteria. Streak the bacteria contaminated loop in the slant

4. Sterilize the mouth of the tube containing slant by exposing it briefly to the flame and cover with a sterile cotton plug.

5. Incubate in an incubator set at the appropriate temperature.

6. Sterilize the loop

In spread plate method, we do different dilutions to avoid bacterial lawn and thus to obtain distinct colonies.

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