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1. At high concentrations, inorganic fluoride inhibits enolase. In an anaerobic

ID: 3166803 • Letter: 1

Question

1. At high concentrations, inorganic fluoride inhibits enolase. In an anaerobic system that is metabolizing glucose as a substrate, which of the following compounds would you expect to immediately increase in concentration following the addition of fluoride?

A. 2-phosphoglycerate.

B. Glucose.

C. 3-phosphoglycerate

D. Phosphoenolpyruvate

E. Pyruvate

2. Suppose you have 1X10 -6 moles of radioactive ATP where only the alpha phosphate group is radioactive (i.e. P-P-*P-suger-base, where the * indicates the radioactive phosphorous atom). If you add all the radioactive ATP to a tube containing hexokinase and 1X10 -6 moles of glucose, what percentage of phosphorylated glucose molecules would you expect to be radioactive after an appropriate amount of incubation?

A.

100%

B.

0%

C.

50% because at equilibrium the concentration of glucose and glucose-6-phosphate will be equal

D.

Impossible to tell

E.

Somewhere between 50-100% because the equilibrium does favor glucose-6-phosphate, but some glucose and ATP will remain.

3.   The fate of pyruvate produced during glycolysis depends primarily on the availability of:

A.

NAD+ to keep the pathway going.

B.

Diatomic oxygen.

C.

ADP for conversion to ATP.

D.

Coenzyme A for further metabolism of pyruvate.

E.

Inorganic phosphate for the synthesis of ATP.

4.   The anaerobic conversion of 1 mol of glucose to 2 mol of lactate by fermentation is accompanied by a net gain of:

A. 1 mol of ATP.

B. 1 mol of NADH.

C. 2 mol of ATP.

D. 2 mol of NADH.

E. None of the above

5.   Starting from glucose, UTP and ATP, how many high-energy phosphate bonds are broken to add glucose to a glycogen molecule?

A.

1

B.

2

C.

3

D.

4

E.

The answer cannot be determined form the information given

A.

100%

B.

0%

C.

50% because at equilibrium the concentration of glucose and glucose-6-phosphate will be equal

D.

Impossible to tell

E.

Somewhere between 50-100% because the equilibrium does favor glucose-6-phosphate, but some glucose and ATP will remain.

Explanation / Answer

1.the correct answer is 2-phosphoglycerate

2.somwhere between 50 - 100 %

3.availability of diatomic oxygen

4.2 mol of ATP

5.3 phoshate bonds are broken