4. You are given two enzymes: \"??-ase\", which catalyzes the interconversion of
ID: 3166778 • Letter: 4
Question
4. You are given two enzymes: "??-ase", which catalyzes the interconversion of the ? and ? forms, and "By-ase, which catalyzes the interconversion of the ? and ? forms. You do not have an enzyme that can interconvert the ? and ? forms A) Starting from a solution of a-D-glucose, what is the greatest fraction of D-glucose that you can B) Starting from a solution of ?-D-glucose, what is the greatest fraction of D-glucose that you can C) A biotech startup claims to have engineered an enzyme that can directly convert ?-D-glucose convert into the ? form at 300 K? convert into the y form at 300 K? into y-D-glucose but not the other way around. With this enzyme, you should be able to convert a solution of a-D-glucose completely into y-D-glucose. Critique this logic. For both (A) and (B), be sure to state exactly how you would run the reaction, i.e., which enzyme(s) you would add and in what order.]Explanation / Answer
A) When the ??-ase added to the solution of ?-D-glucose, the enzyme converts ?-D-glucose to ?-D-glucose until the equilibrium is reached, since the reaction is reversible. That is the amount of ?-D-glucose and ?-D-glucose will be equal. So,
The fraction of ?-D-glucose = 1/2 = 0.5
Fraction of ?-D-glucose =1/2= 0.5.
B) When the ??-ase and ??-ase are added to the solution of ?-D-glucose, the enzyme converts ?-D-glucose to ?-D-glucose which will be converted to ?-D-glucose until the equilibrium is reached, since the reactions are reversible. That is the amount of ?-D-glucose, ?-D-glucose and ?-D-glucose will be equal. So,
Fraction of ?-D-glucose = 1/3 = 0.33
Fraction of ?-D-glucose = 1/3 = 0.33
Fraction of ?-D-glucose = 1/3 = 0.33
C) When the ??-ase added to the solution of ?-D-glucose, the enzyme converts ?-D-glucose to ?-D-glucose continuously irrespective of equillibrium, since the reaction is irreversible. That is all the ?-D-glucose will be converted to ?-D-glucose in suitable condition. So,
The fraction of ?-D-glucose = 1
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