2. The use of reassortant viruses can be used experimentally to determine which

Question

2. The use of reassortant viruses can be used experimentally to determine which gene segment (and its corresponding viral protein) is associated with a specific pathogenic phenotype. In the following example, reovirus T1L was mixed with reovirus T3D to determine the mode of spread to the central nervous system (CNS) after injection into the foot of mice. As shown in the table below, T3D spreads exclusively by a neural route (i.e., traveling nerve-to-nerve) while T1L spreads through the bloodstream before crossing the blood/brain barrier Origin of gene segment Virus VirusIsolate Pattern of in vivo Spread TIL L?L?L L L L L L L L Bloodviremia Parent strain R1 R2 D R3 L L L L L L L L L D?Bloodviremia R4 D Reassortant | R5 | DIDI Variants R6 R7 R8 R9 Neuronal Neuronal (a) Based on the above data, which genome segment was found to be responsible for the pathway of virus spread within the mice? [3 pts.] (b) How do you think the protein encoded by the above indicated gene segment influences virus spread? Explain your reasoning. [4 pts.]

Explanation / Answer

Ans-A according to the table the o1 of S segment is responsible for the pathway of spread choose by the virus.

Because on all the other positions there are other protein present other than the responsible protein but at this site only the responsible protein are present.

Ans-B the reason behind this is that there are only two proteins i.e. D and L. On other gene segments they may be replaced by one another but at the o1 of S segment only that protein is present which represent it's specific spread pathway. In the blood viremia it is L and in neuronal it is D.

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