1. Shortly after the discovery of positive-sense RNA viruses such as poliovirus,
ID: 3166116 • Letter: 1
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1. Shortly after the discovery of positive-sense RNA viruses such as poliovirus, researchers attempted to discover more about the mechanisms of how these viruses replicated their genomes. A famous virologist named David Baltimore made a crucial discovery in this field by making use of a drug inhibitor called Actinomycin D. This molecule can be used in the laboratory to inhibit the synthesis of cellular mRNA transcription. (a) If this drug was added to poliovirus-infected cells, what do you suppose would happen to viral RNA synthesis and genome replication? Explain your reasoning. 12 pts.] viral RNA synthesis and genome replication? Explain your reasoning. 12 pts.] viral RNA synthesis and genome replication? Explain your reasoning. 12 pts.] (b) If this drug was added to reovirus-infected cells, what do you suppose would happen to (c) If this drug was added to herpesvirus-infected cells, what do you suppose would happen toExplanation / Answer
Ans a) If we add actinomycin-D at low concentration, it might have no effect or slightly inhibit the growth of the cells. On addition of the same, it does not really inhibit the RNA synthetase activity in vitro and neither have any major impact on the DNase enzyme activity. It might however lead to inhibition of protein and RNA synthesis and it might lead to certain morphological changes in the poliovirus infected cells.
Ans b) On adding the actinomycin-D to reovirus infected cells, the reproduction of the reovirus is most likely to be inhibited and it requires low concentration to actually hinder and interrupt the growth. It largely leads to the inhibition of the cellular DNA but does not necessarily inhibit the RNA or protein formation.
Ans c) If actinomycin D was added to the Herpes Virus infected cells, it mostly was seen to add the concentration of the DNA polymerase activity and might lead to increase in the genome replication. However the enhancement of the endogenous RNA polymerase occurs slowly and steadily
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