1. Sequential binding of RNA polymerase II-TFIIF complex, TFIIE, and TFIIH compl
ID: 168784 • Letter: 1
Question
1. Sequential binding of RNA polymerase II-TFIIF complex, TFIIE, and TFIIH completes ___________________ formation.
none of the above
2. The underlying principle behind ________________ assays is that binding a protein to radioactive DNA fragment will produce a complex that will migrate more slowly than the free DNA fragment when both are subjected to gel electrophoresis.
pre-initiation complex TF recognition element pre-elongation complex TATA binding complexnone of the above
2. The underlying principle behind ________________ assays is that binding a protein to radioactive DNA fragment will produce a complex that will migrate more slowly than the free DNA fragment when both are subjected to gel electrophoresis.
primer extension gel mobility shift SI mapping microarrays none of the aboveExplanation / Answer
Answer:
1. (a) pre-initiation complex
Sequential binding of RNA polymerase II-TFIIF complex, TFIIE, and TFIIH completes pre-initiation complex formation.
The core promoter is the site for assembly of the transcription preinitiation complex, which includes RNA pol II and the general transcription fctors TBP, TFIIB, TFIIE, TFIIF, and TFIIH.
Order-of-addition experiments demonstrated that Pre-initiation complex (PIC) assembly is nucleated in vitro by TBP binding to the TATA element followed by binding of TFIIB, RNA pol II-TFIIF, TFIIE, and TFIIH.
2. (b) gel mobility shift
The underlying principle behind gel mobility shift assays is that binding a protein to radioactive DNA fragment will produce a complex that will migrate more slowly than the free DNA fragment when both are subjected to gel electrophoresis.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.