The creation of transgenic crop plants using recombinant DNA methods involves th
ID: 3165750 • Letter: T
Question
The creation of transgenic crop plants using recombinant DNA methods involves the transfer of just one gene or a small number of genes to the plants, in contrast to classical breeding methods in which hundreds or even thousands of genes are transferred at once. Explain why this is true. If fewer genes are transferred during the creation of transgenic crops, why are some people afraid that they are dangerous? What is the difference between gamete intrafallopian transfer (GIFT) and intracytoplasmic sperm injection (ICSI)?Explanation / Answer
Ans. a)
As, Genetically Modified plants contain artificially inserted gene(s) from another unrelated plant or from a completely different species via genetic engineering techniques, people are afraid that they are dangerous because of the following possibilities -
1- the protein encoded by the foreign gene inserted into the plant might be allergic or toxic to the consumers.
2- GM plants which contain insecticidal transgenes may also affect non-target organisms and may reduce their population as well.
3- the transgenic plants which are resistant to many factors, might become invasive and it would be difficult to control their growth when released into the environment.
4- wide spread cultivation of GM crops with pest or disease resistance will impose intense selection pressure on pest and pathogen populations to adopt to the resistance mechanism.
5- GM crops will lead to loss of biodiversity by replacing wild type populations.
Ans. b)
in Gamete intrafallopian transfer (GIFT), Mature ova are removed from large follicles from ovary and are inserted by laparoscopy into one of the woman’s oviducts and the sperm sample is also placed into the oviduct with the ova. Fertilization then occurs in the fallopian tube followed by implantation.
in intracytoplasmic sperm injection (ICSI), sperm is directly injected into the cytoplasm (interior) of the egg. so, in ICSI fertilization occurs outside the body and then the embryo is transferred into the women for implantation.
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