Bios Sample Lab Quiz Fill in the blank (1 pt each blank) 1. et ren t o Lol u s _
ID: 3165207 • Letter: B
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Bios Sample Lab Quiz Fill in the blank (1 pt each blank) 1. et ren t o Lol u s _ is a genus associated with ? hemolysis 2. In the genetic exchange experiment, thestrain was able to grow on minimal media with streptomycin following the conjugation. is a genus that is isolated using chocolate agar was a genus we were looking for when we streaked the mannitol salt 3. agar plate. Short Answers 1. Why did you stab the blood agar after inoculating it? (2pts) 2 What is the composition of chocolate agar and why might a species require this agar? (2pts) 3. In gne sentence, describe the purpose of the ELISA test we are doing in lab. (2pts)Explanation / Answer
3. Chocolate agar is used for isolation and cultivation of fastidious microorganisms such as Neisseria and Haemophilus species.
4. Phenylethyl Alcohol agar is simple selective medium designed to inhibit gram negative organism . Mannitol salt agar was developed to favor growth of staphylococcus and to differentiate pathogenic from non pathogenic members of the genus.
1. The easiest way to provide environmenta favorable to streptolysins on blood agar is to perform what is calles as streak- stab technique. In this procedure the blood agar plate is streaked for isolation and then stabbed with a loop. The stab ancourages streptolysin activity due to the reduced oxygen concentration of the subsurface environment.
2. It is a varient of blood agar plate containing red blood cells that have been lysed by slowly heating at 80 degree. Chocolate agar is used for growing fastidious respiratory bacteria such as Haemophilus influenzae and Neisseria menningitidis.
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