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Table 10.1: Gram stain results after the application of each reagent. Step Prima

ID: 3164518 • Letter: T

Question

Table 10.1: Gram stain results after the application of each reagent. Step Primary stain Mordant Reagent Crystal violet Gram's iodine Gram-positive Results Purple Purple Purple Purple Gram-negative Results Purple Purple Clear/Colorless Decolorizing Acetone-alcohol Counterstain Safranin Pink/Red u begin the procedure, you should be aware of some common sources of errors in the Gram efore yo taining technique. That way, you can be careful to try to avoid them. They are: . The loop was too hot when you made the smear. . Excessive heat was applied during the heat fixing. . The decolorizer was left on the smear too long. . The culture was too old (more than 48 hours). The smear was too thick. rocedure 10.2A: Preparing slides efore you begin, remove all personal items and books from your lab space. Wipe the desk horoughly with disinfectant. This will help remove any transient bacter ia and help prevent environment. Place a staining rack over your s ontamination of your slides with bacteria from the

Explanation / Answer

4) A) If you used safranin as the primary stain and crystal violet as the secondary stain. Gram-positive cells would stain a purple color and Gram-negative cells would stain a purple color.

B) If you forgot to add the acetone-alcohol decolorizing agent. Gram-positive cells would stain a purple color and Gram-negative cells would stain a purple color.

C) If you left the acetone-alcohol decolorizing agent on the cells for 60 seconds instead of 3 seconds. Gram-positive cells would stain a clear / colorless color and Gram-negative cells would stain a clear / colorless color.

5) Human cells have no cell wall, so the primary stain would be easily removed by alcohol. Safranin would bind to remaining structures with a negative charge. Therefore, we will see pink/red color.

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