6. If a double stranded DNA molecule is composed of 31% deoxyguanosine, then wha

Question

6. If a double stranded DNA molecule is composed of 31% deoxyguanosine, then what is the percentage of deoxycytidine, deoxyadenosine, and deoxy thymidine 7. Double stranded DNA has how many grooves? What are they called? Can you explain WHY these grooves are different sizes (this is a very difficult question. You have to look at the symmetry or lack of symmetry in a base pair and then think about what happens when you stack base pairs)? If you can't answer this question, don't lose sleep. Go to this website htt 8. You have a vial of double stranded DNA in an aqueous solution that is composed 100% of only A-T base pairs. You have a second vial of double stranded DNA in an aqueous solution that is composed 100% of only G-C base pairs. Double stranded DNA can be denatured into single stranded DNA by increasing the temperature. Which vial of DNA will denature at a lower temperature? Why?

Explanation / Answer

7

double helical nature of DNA, the molecule has two asymmetric grooves. One groove is smaller than the other. This asymmetry is a result of the geometrical configuration of the bonds between the phosphate, sugar, and base groups that forces the base groups to attach at 120 degree angles instead of 180 degrees. The larger groove is called the major groove while the smaller one is called the minor groove.

Since the major and minor grooves expose the edges of the bases, the grooves can be used to tell the base sequence of a specific DNA molecule. The possibility for such recognition is critical, since proteins must be able to recognize specific DNA sequences on which to bind in order for the proper functions of the body and cell to be carried out. As you might expect, the major groove is more information rich than the minor groove. This fact makes the minor groove less ideal for protein binding.

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