Of those that recognize offset sites and generate staggered cuts, which of these

Question

Of those that recognize offset sites and generate staggered cuts, which of these cuts cannot be converted to flush ends by the action of DNA polymerase?

Specificities of some type restriction systems Enzyme Bacterial Source Restriction and Modification SiteA Bacillus am H GIGATcc BamHI Al GATCT B. globiggi GIAATTC EcoRI Escherichia coli RY13 E. coli R245 EcoRI CCIGG GGIcc Hael Haemophilus aegyptius GAcGCNNNNNI CTGcGNNNNNNNNNNN. H. gallinarum GCGIC Hhal H. haemolyticus HindMI GTPyl PuAC H. influenzae Rd ANAG CTT H. influenzae Rd GNANTC Hinfl H. influenzae Rf GTTNAAC Hpa H. parainfluenzae ClCGG Hpa H. parainfluenzae CICGG Moraxella sp. GcIGGCCGC Nocardia rubra Notl GAGTcNNNN CTCAG NNNNNN Pel Pseudomonas lemoignei CTGCANG Pst Providencia stuartii GITCGAC Streptomyces albus G CCCIGGG Smal Serratia marcescens Sb TICTAGA Xbal Xanthomonas badrii

Explanation / Answer

The DNA strands that cannot be made into flush strands are BglII,EcoRII, Hinf1, Ple1 and Sma1.

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