l0 . Complete the data table below based on the extracted DNA Title. Calculation

Question

l0

. Complete the data table below based on the extracted DNA Title. Calculation Unknunin )Nn Concen hohe Ulm? the DNA conc Extracted DNA [volume used in ml) 0.5 Ab @ Extrapolated Calculated ug DNA ?g/mL oxy s and the concentration in micrograms/mL in which one used ½ mL of Calculation of DNA in microgram extracted DNA. [1 mark] Calculatio extracted DNA n of DNA in micrograms and the concentration in micrograms/mL in which one used 1 mL of [1 mark] Conclusion - in sentence format, state the conclusion(s) of this lab exercise on the quantification of DNA using diphenylamine reaction 9. [2 marks]

Explanation / Answer

For diphenyl amine method DNA quantitation done at 595 nm or 600 nm.

In first the ½ ml of volume is used that you have already done.

In second box the volume 1 ml that you have already done.

For question no 9.

Here the concentration of extracted DNA is depend volume that used. Both the DNA having same concentration that is represented in ug/ml but amount of DNA is different that represent in ug.

This problem is basically told about the role of volume to determine the concentration.

If you have any other query please ask.

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