EXPLAIN EACH ANSWER PLEASE. 3. (6 points) Consider five proteins with the proper

Question

EXPLAIN EACH ANSWER PLEASE.

3. (6 points) Consider five proteins with the properties described in the following table Answer the questions below about the proteins and justify your answer Protcin Subunit mass (Da) Native mass (Da) 10.000 15,000 8 20,000 25,000? 30,000,2 40,000 20,000 20,000 75,000 60,000 a) Which protcin wil clute last from a gel filtration chromatography b) Which proteinwill migrate the slowest in n SDS-PAGE electrophoresis c) What is likely the quatcrnary structurc of protcin D on thc basis of the data in the tablc.

Explanation / Answer

Protein that will elute last from a gel filteration technique-

Consider the separation of a mixture of glutamate dehydrogenase, lactate dehydrogenase, serum albumin , ovalbumin, and cytochrome on a gel filtration column packed with Bio-Gel . When the protein mixture is applied to the column, glutamate dehydrogenase would elute first because it is above the upper fractionation limit. Therefore it is totally excluded from the inside of the porous stationary phase and would elute with the void volume(V0). CYTOCHROME is the protein which will be getting eluted at last from fractional distillation. The other proteins would be partially included and elute in order of decreasing molecular weight.

Protein that will migrate slowest in an SDS PAGE electrophoresis

Sodium dodecyl sulfate is a protein that will migrate slowest in an SDS PAGE

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