Lesson 2 Restriction Digestion of DNA Samples Review Questions 1. Before you inc

Question

Lesson 2 Restriction Digestion of DNA Samples Review Questions 1. Before you incubated your samples, describe any visible signs of change in the contents of the tubes containing the DNA after it was combined with the restriction enzymes 2. Can you see any evidence to indicate that your samples of DNA were fragmented or altered in any way by the addition of EcoRUPsl? Explain 3. In the absence of any visible evidence of change, is it still possible that the DNA samples were fragmented? Explain your reasoning. 4. (Answer the next day) After a 24 hour incubation period, are there any visible clues that the restriction enzymes may have in some way changed the DNA in any of the tubes? Explain your reasoning

Explanation / Answer

Answer 1

DNA + EcoRI/PstI enzyme mix:No visible change apparent in the tubes.

Answer 2

No. No visible change apparent in the tubes.

Answer 3

Yes. . Enzymesmay have cut the DNA. but the changes may not be visible because of chemically changed

Answer 4

No. The reactions are at the molecular level and too small to be seen.No visible change apparent in the tubes but the enzymes may have cut theDNA.

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