1 a?The enzyme lysozyme catalyzes the hydrolysis of glyosidic bonds of peptidogl
ID: 300786 • Letter: 1
Question
1 a?The enzyme lysozyme catalyzes the hydrolysis of glyosidic bonds of peptidoglycans, a molecule found in the cell wall of some bacteria. Consider the three-dimensional model of the enzyme lysozyme (A) and a map of the location of the important binding and catalytic amino acids that participate at the active site (B) and answer the following questions.
1 b?) Research has shown that amino acid 35 (yellow), a glutamic acid, is critical for catalysis. Other studies have found that amino acids 62 (pink) and 63 (purple), both tryptophan, are critical for substrate binding. Use this information and figure 4.2 in your book to answer the following questions: Do you think changing amino acid 35 from an aspartic acid to a lysine will affect substrate binding, catalysis, both, or neither? Explain your answer (200-word limit)
Explanation / Answer
1. Active site of the enzyme is the specific area where substrate attach and performed chemical reaction. The action site of the enzyme is comprised of specific amino acid that are needed to performed chemical reaction. These specific residues form the temporary bond with the enzyme. Active site of the enzyme form a small area in the enzyme but is the most important part of the enzyme as it perform the catalysis of enzyme. Active site of the enzyme have five -six amino acid and remaining amino acid made the structural part of enzyme
In the 3d structure of the given enzyme -substrate, if amino acid 35 is changed from glutamic acid to lysine, both substrate binding and catalysis changed. This is because, the glutamic acid is an acidic amino acid which is substituted by lysine which is a basic amino acid. This change of amino acid form acidic nature to basic nature, will result in change of 3d structure of protein due to which substrate is not able to make a bond with active site and hence catalysis of substrate is also affected.
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