PART A:) A research team is studying regulatory sequences for genes that are imp

Question

PART A:) A research team is studying regulatory sequences for genes that are important for control of the cell cycle. The researcher would like to construct a DNA library that contains these regulatory sequences for all of the cell cycle genes. What type of library what they construct, a genomic or cDNA library?
PART B:) The same research team is also interested in determining if the mRNAs for cell cycle genes are expressed differently in different tissues (e.g. normal vs. cancer tissues). What type of library will help the researchers compare gene expression between two different tissue types, a genomic or cDNA library? PART A:) A research team is studying regulatory sequences for genes that are important for control of the cell cycle. The researcher would like to construct a DNA library that contains these regulatory sequences for all of the cell cycle genes. What type of library what they construct, a genomic or cDNA library?
PART B:) The same research team is also interested in determining if the mRNAs for cell cycle genes are expressed differently in different tissues (e.g. normal vs. cancer tissues). What type of library will help the researchers compare gene expression between two different tissue types, a genomic or cDNA library? PART A:) A research team is studying regulatory sequences for genes that are important for control of the cell cycle. The researcher would like to construct a DNA library that contains these regulatory sequences for all of the cell cycle genes. What type of library what they construct, a genomic or cDNA library?
PART B:) The same research team is also interested in determining if the mRNAs for cell cycle genes are expressed differently in different tissues (e.g. normal vs. cancer tissues). What type of library will help the researchers compare gene expression between two different tissue types, a genomic or cDNA library?
PART B:) The same research team is also interested in determining if the mRNAs for cell cycle genes are expressed differently in different tissues (e.g. normal vs. cancer tissues). What type of library will help the researchers compare gene expression between two different tissue types, a genomic or cDNA library?

Explanation / Answer

PART A. Genomic. As the regulatory sequences are inter-genic sequences (between gene sequences), most likely, upstream elements of the genes, a cDNA library will not cover these elements. Therefore, a genomic library is required.

PART B. cDNA. As the researcher wants to monitor the expression of different genes in different tissues, he has to extract the mRNAs and convert them to cDNAs and construct a library.

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