Unlike DNA replication in the cell, PCR uses heat to separate DNA strands. (T/F)

Question

Unlike DNA replication in the cell, PCR uses heat to separate DNA strands. (T/F) Restriction endonucleases are enzymes that cut DNA at specific double stranded sequences (T/F) Taq polymerase, an enzyme found in thermophilic bacteria, is used to replicate DNA in PCR. (T/F) Bacteriophages, viruses that infect bacteria, can be used to deliver recombinant DNA into host cell. (T/F) Antibiotic resistance genes are often included in cloning vectors to serve as selection markers. (T/F) Restriction fragment Length Polymorphisms (RFLPs) can be used to generate "DNA fingerprints." (T/F) Green Fluorescent Protein (GFP) is an example of a "reporter protein" naturally found in bacteria. (T/F) Reverse transcriptase is an enzyme that generates "sticky ends." (T/F) A polylinker is a segment of a plasmid vector that contains multiple restriction enzyme sites. (T/F) bacterial colonies containing recombinant DNA (T/F)

Explanation / Answer

Answers :

32. This statement is TRUE. As PCR uses 94 0C for the purpose of denaturation prior to amplification of DNA fragments.

33. This statement is TRUE. As restriction enzymes cut at both the strands of double stranded DNA molecule.

34. This statement is TRUE. Unlike DNA polymerase which is thermolabile, Taq polymerase which is used in PCR is thermostable enzyme which is obtained from thermophilic bacterium Thermus aquaticus.

35. This statement is TRUE. Since bacteriophage vectors such as lambda phage and coliphage are used as vectors in recombinant DNA technology.

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