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12.15 Atgal batch growth kinetics Chlorella sp. algae are used to produce lipids

ID: 285741 • Letter: 1

Question

12.15 Atgal batch growth kinetics Chlorella sp. algae are used to produce lipids for the manufacture of biodiesel. The kinetics of algal growth are determined in batch culture under phosphate-limiting conditions at an illumination intensity of approximately 60 o1 m-2 s-1 and a photoperiod of 14 h light:10 h dark. The following results are obtained from five different cultures at temperatures from 19°C to 28.5°C, including data from a replicate culture at 25°C T= 19°C T = 20° C T= 25°C T= 25°C T-28.5°C Cell Cell Cell 2 Cell Cell Time Concentration Time Concentration Time Concentration Time Concentration Time Concentration (days)(cells l-1) (days) (cells l-1) (days) (cells l-1) (days) (cells l-1) (days) (cells ri) 0 0.2 1.87×105 0.5 3.9981 0.5 2.44 105 1.0 6.97 × 105 1.0 612×105 1.4 1.34 × 106 2.1 4.88 × 106 2.1 766 × 106 2.1 903 × 106- 1.8 1.72 × 106 2.1 3.98 × 106 2.2 3.92 × 106 3.5 4.12×10 2.5 701 × 106 3.0 1.75 × 10, 3.8 4.70×107 . 1.35 × 105 2.95 × 105 1.02 × 106 1.34 × 106 0 1.0 1.5 2.6 1.30 × 105 1.87 × 105 2.44 x 105 6.12×105 1.30 × 10 1.30 × 105 1.35 × 10 05 0.5 2.58 × 105 0.5 9.49 × 105 , 2.91×106 1.0 1.5 1.0 1.5 1.14 × 106 1.5 , , 1.77 × 107 2.6 6.26 × 107 - - 2.6 . 7 3.8 2.03 × 10s 3.8 2.96 × 10s - _ (a) Do the cultures exhibit exponential growth at each of the temperatures tested? (b) E valuate the maximum specific growth rates and doubling times for each of the five algal cultures

Explanation / Answer

Ans-1)- Yes, Exponential growth is observed at each step.

Ans-2)-Max.specific growth rate = 14 h-1

Doubling time= 7 hours

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