Use the following abstract to answer questions 19-22 (5 pts each) PLOS ONE 8(7)

Question

Use the following abstract to answer questions 19-22 (5 pts each) PLOS ONE 8(7) 2013 Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 ina Chinese Soybean Cultivar Jiging Zhang. Changjan Xia, Canxing Duan, Suli Sun, Xiaoming Wang. Xiaofei Wu Zhendong Zhu Resistance to Phytophthora sojae isolate PsMC1 was evaluated in 102 F2:3 families derived from a cross between the resistant soybean cultivar Wandou 15 and the susceptible cultivar WWliams and genotyped using simple sequence repeat (SSR) markers. The segregation ratio of resistant, segregating, and susceptible phenotypes in the population suggested that the resistance in Wandou 15 was dominant and monogenic. Twenty-six polymorphic SSR markers were identified on soybean chromosome 17 (Molecular linkage group D2; MLG D2), which were linked to the resistance gene based on bulked segregation analysis (BSA). Markers Sattwd15-24/25 and Sattwd15-47 flanked the resistance gene at a distance of 0.5 cM and 0.8 cM, respectively. Two cosegregating markers. Sattwd15-28 and Sattwd 15-32, were also screened in this region. This is the first Rps resistance gene mapped on chromosome 17, which is designated as Rps10. Eight putative genes were found in the mapped region between markers Sattwd15-24/25 and Sattwd15-47. Among them, two candidate genes encoding serine/threonine (Ser/Thr) protein kinases in Wandou 15 and Wiliams were identified and sequenced. And the differences in genomic sequence and the putative amino acid sequence respectively, were identified within each candidate gene between Wandou 15 and Williams. This novel gene Rps10 and the linked markers should be useful in developing soybean cultivars with durable resistance to P. sojae. Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar Zhang J. Xia C, Duan C, Sun S, Wang X, et al. (2013) Identification and Candidate Gene Analysis of a Novel Phytophthora Resistance Gene Rps10 in a Chinese Soybean Cultivar. PLOS ONE 8(7): e69799. dok: 10.1371/journal.pone.0069799 What is the phenotype of interest? An F2:3 population was created. What are the names of the two parents? This abstract states that the gene conferring resistance is dominant. Which of the following segregation ratios would be expected in the F2 generation? A 100 resistant to 300 susceptible plants B 287 resistant to 98 susceptible plants C 100 resistant to 100 susceptible plants 0 D 6 resistant to 30 susceptible plants E None of the above or This abstract states that the gene conferring resistance is dominant. Which of the following segregation ratios would be expected in the F1 generation? A 100 resistant to 300 susceptible plants B 287 resistant to 98 susceptible plants . C 100 resistant to 100 susceptible plants D 6 resistant to 30 susceptible plants E None of the above 211

Explanation / Answer

1) Phenotype of interest is Disease resistance aginst Phytophthora sojae infecting soybean.

2) Names of two parents for F2:3 ipopulation are: Wandou 15 ( resistant cultivar) and Williams (succeptible cultivar)

3) As the gene confering resistant is dominant, we should expect 3:1 ratio (as in monohybrid ratio in F2 population) between Resistance : Succeptible cultivars. Among all answers option A gives approximately right ratio of 3:1 which is the right answer ( Note that none of the genetic crosses results in exact ratios).

4) Since it is mentioned that the phenotype is monogenic , in F1 of monogenic cross we expect 100% of heterozygous plants with dominant trait ,that is in F1 population all plants will be resistant to disease. so Option E none of these is the correct option.

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