40) If the length of the resulting real-time PCR products are identical use the

Question

40) If the length of the resulting real-time PCR products are identical use the melt curve data below to predict which of the following peaks would correspond to the PCR product that has the highest percentage of G-C basepairs in it. 0.017 Base TemperaturnC) A) The peak labeled A would have the greater G-C ba B) The peak labeled B would have the greater G-C basepairing. C) The peak labeled C would have the greater C-C base pairing. D) The peaks labeled A and B would have the greater G-C basepairing E) The peaks labeled C and B would both have the greatest G-C basepairing. g.

Explanation / Answer

Option A is correct as higher GC content results in peak of Tm curve.

Dissociation curve analysis distinguishes PCR products on the basis of Tm. Since the Tm is characteristic of the GC content, length and sequence of a DNA product, it is a useful tool in product identification. This method is therefore commonly used to establish that the correct target DNA has been amplified and is most commonly used at the end of PCR assays to check for non-specific products and/or contamination.  

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