Question 2 (20 pts) In part based on your Reading \"Basics of Microscopy\" but a

Question

Question 2 (20 pts) In part based on your Reading "Basics of Microscopy" but also information from your book. Microscopy has always been central to histology and advancements in histology are often linked to advancements in imaging. (I) describe the main types of microscopes commonly used in microscopy. (II) explain why there is a physical limit to the resolution that can be accomplished with a light microscope and (III) provide an example each of a structure/animal/plant/microbe that can best be viewed with (i) light microscope; (ii) scanning electron microscope; (iii) transmission electron microscope; (iv) atomic force microscope; (v) confocal microscope. You are welcome to use outside resources while answering this question -just avoid any suspicion of plagiarism!

Explanation / Answer

1) The main types of microscopes used in microscopy are listed below,

2) There is physical limit to resolution by light microscope because of wavelength of the light used and the resolution power of the lens used.

3) Light microscope - malarial parasites

Scanning electron microscope - bacteriophage

Transmission electron microscope - pollengrains

Atomic force microscope - identification of individual surface atoms

Confocal microscope - identification of filamentary fungal elements in corneal stroma in keratomycosis

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