1. Hindill is a restriction endonuclease isolated from E. coli H. influenzae H.
ID: 282127 • Letter: 1
Question
1. Hindill is a restriction endonuclease isolated from E. coli H. influenzae H. pylorie B. subtilis D. 2. T-DNA fromintegrates into the plant genomic DNA causing abnormal proliferation of cells. E. coli A. H influenzie A. tumefaciens S aureus . To amplify a DNA fragment by PCR, which one of the following will you NOT need? Taq polymerase Buffer D. Restriction endonulcease E. Primers F. Template DNA C. dNTPs DNA is synthesized by the condensation of nucleotides catalyzed by RNA polymerase A. DNA polymerase C. DNA Topoisomerase D DNA ligase Typically a vector plasmid contains - Replication origin Antibiotic resistance gene Multiple cloning site All of the above A. B. D between In a DNA molecule, two strands are held together through omplementary bases A. Covalent bonds B. hydrogen bonds phosphodiester bonds D. peptide bonds elting/ A. denaturation of double stranded DNA can be done in vitro using Heat B. DMSO (Dimethyl sulfoxide) D All (A-C) are true Below is a skeleton diagram of a pentose sugar. Circle the correct letter in the diagra Formamide h question numberExplanation / Answer
1) The answer is B.It cuts at AAGCTT.
2) The answer is c. It is the tumor inducing DNA of Agrobacterium tumefaciens.It is transferred into the plant genome due to which plant grows in tumor and produces food source for bacterium.
3) The answer is D.As restriction endonuclease is needed to cut the DNA fragment while in PCR there is not need t cleave the DNA.
4) The answer is a (DNA polymerase).This enzyme add up the nucleotides to the growing chain and makes DNA.
5) The answer is D (ALL OF THE ABOVE).Plasid is an extrachromosomal circular DNA which is autoreplicable having resistant gene.
6) The answer is B (hydrogen bond).There is hydrogen bond between two strands of DNA which runs antiparallel.Adenine pairs with thymine by two hydrogen bond and cytosine pairs with guanine with three hydrogen bond.
7) The answer is A (heat).Heat breaks hydrogen bonding between two strands of DNA that runs antiparallel.
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