Genetics. Help ASAP please. Part A You have constructed four different libraries

Question

Genetics. Help ASAP please. Part A You have constructed four different libraries: a genomic library made from DNA isolated from human brain tissue, a genomic library made from DNA isolated from human muscle tissue, a human brain cDNA library, and a human muscle cDNA library Assuming the human genome is 3 x 10° bp and that the average insert size in the genomic libraries is 100 kb, how frequenty will a clone representing myostatin be found in the genomic library made from muscle? Express your answer as a probability You wish to clone the human gene encoding myostatin, which is expressed only in muscle cells. /60,000 Submit Previous Answers Request Answer XIncorrect; Try Again; 5 attempts remaining Part B will a clone representing myostatin be found in the genomic library made from brain? Express your answer as a probability. Submit X Incorrect; Try Again; 5 attempts remaining Part C How frequenty will a clone representing myostatin be found in the cDNA lbrary made from brain? Express your answer as a probability Submit Next> Provide Feedback

Explanation / Answer

Part A.

1/30000. Genome size is 3x109 bp while clone size is 100 kb or 105 bp. So frequency will be 1/[(3x109)/105] = 1/30000.

Part B.

1/30000. Since sequences in the genomic libraries overlap completely.

Part C.

0. Myostatin is expressed in muscles and not in brain, so cDNA will not contain myostatin gene.

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