Based on the article I need a Prelab for this article due tomorrow. I don\'t kno

Question

Based on the article I need a Prelab for this article due tomorrow. I don't know how to do it and it's last min. so I hope someone can help me. I need it to be at least a page or 1 1/2 oh. here's an example. (like a how to) with purpose, steps (protocol), and small conclusion.

Also Pre-lab format will need to include an explanation of the purpose of (why am I doing it) and then a bulleted list of the protocol (how are you doing it).

5 CELLS 5 Jerome A. Montvilo Wir haben gesehen dass alle Organismen aus wesentiich gleichen Theilen, namlich aus Zelien zusammengesetzt sind. (We have seen that all organisms are assembled by essentially the same parts, namely by cells.) Theodor Schwann, 1839 Long ago it became evident that the key to every biological problem must finally be sought in the cell, for every living organism is, or at some time has been, a cell. E. B. Wilson, 1925 The living cell is to biology what the electron and the proton are to physics. Apart from cells and from aggregates of ceils there are no biological phenomena. Alfred North Whitehead, 1925 1. INTRODUCTION The study of cells can be traced back to the work of Robert Hooke in the 1600s who first discovered empty spaces in slices of cork. He called these empty spaces, which were surrounded by walls, "cells". Today we know that those empty spaces are usually filled with the materials a cell needs to remain alive. When Matthias Schleiden, a botanist, and Theodor Schwann, a zoologist, first popularized the so-called "Cell Theory" in the early 1800s, it was a remarkable generalization: “All living things are made from cells." As then, we even now hold that generalization to be true. We have learned quite a bit more about cells and cell structure since then, and we know even more about how the cells work. In fact, the Cell Theory has been expanded to include the principle that cells are the basic structural and functional units of living things. Thebasic components of all cells are an outer cell membrane (also called a plasma membrane) and an internal gel-like material called cytoplasm. Suspended within the cytoplasm may be various structures called organelles. Also found within each cell is its genetic material, DNA. Structurally, cells may be simple or complex. The relatively simple "prokaryotic" cells appear to be little more than a cell wall outside the outer membrane which in turn surrounds a simple cytoplasm with few organelles. The more complex "eukaryotic" cells may or may not have a cell wall (plant cells do, animal cells typically do not) surounding an outer membrane which in turn surrounds a complex cytoplasm consisting of many organelles, the main one of which is the nucleus. (The term "eukaryotic" refers to the fact that these cells have a "true nucleus" in which genetic material is stored. Prokaryotic cells lack a nucleus.) It is generally held that prokaryotic cells developed early in the evolution of life on Earth and, through a process of endosymbiosis, contributed to the development of eukaryotic cells Based, with gratitude, on work done by Charles V. Foltz. Copyrigh! 2014. At rights reserved. Version printed 757N4 RIC fo Bil 100: Fundemental Concepts ef Bialogy, Cells Page 5-1

Explanation / Answer

Prelab;

Introduction to visualization and study of various types of cells under Light Microscope.

Purpose:

In order to understand the working and functioning of cells, it is vital to visualize the cells and its organelles by directly or staining the cells and studying them. In today's lab-wide range of cells like the sea star ovum and buccal epithelial cell to study the nucleus and nucleoplasm, onion cells to study the cell wall, banana cells and plant cells to study cell wall and organelles like plastids(amyloplasts), vacuoles, bacteria to study and compare its size and morphology as upon eukaryotic cells.

Protocol;

1) Clean the micro slides and coverslips properly and keep stains like methylene blue, safranin ready.

2) samples should be prepared one by one as mentioned in the information sheet.

3) The microscopes playing a vital role in the visualization needs proper maintenance and cleaning with acetone dipped tissue paper.

4)The prepared slides should properly be kept and adjusted under the microscope at 4X to locate the cells, further magnification can be done by just rotating the eyepiece and not disturbing the position of the slide.

5)the observed image should be captured using a good megapixel camera or could be jotted on a practical record book for future reference.

Conclusion:

Visualization and study of cells can be performed using the above-mentioned technique which enables us to widen the practical knowledge of the subject.

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