Determine the amounts/volumes of the chemicals needed to prepare the following s

Question

Determine the amounts/volumes of the chemicals needed to prepare the following solutions (w' weight: v' volume; 'mw'- molecular weight) 1, 50 ml of 0.9% (w/v) saline (NaCl) [you will need this solution as a 2, 30 ml of 50% glycerol (v/v-in distilled water) stock solution when making 10X gel loading dyel You will need to make 50X TAE so that you can do gel electrophoresis. One of the ingredients is 0.5 M EDTA pH 8.0. EDTA stands for disodium ethylenediamindetratraacetate 2(H2O). This EDTA solution will be provided to you. However, you should know how it was made, and the following two questions address this

Explanation / Answer

1. 0.9% saline :

0.9 g NaCl in 100 ml solution.

0.45 g NaCl in 50 ml solution.

0.45 g NaCl is taken in a measuring jar, add distilled water upto 50 ml. It gives 0.9% saline.

2. 50% v/v glycerol solution:

50 ml glycerol in 100 ml solution.

15 ml glycerol in 30 ml solution.

15 ml glycerol is taken in measuring jar, to it add distilled water upto 30 ml. It gives 50% v/v glycerol solution.

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