The following categories should be addressed for Gardnerella vaginalis: Clinical
ID: 278452 • Letter: T
Question
The following categories should be addressed for Gardnerella vaginalis: Clinical significance of the organism o Is the organism always pathogenic? o Is the organism normal flora? If yes, where on the body is it found? o Are there specific populations that are vulnerable? If yes, what are they? Common infections What diseases or specific types of infection are associated? o Special Media/Growth Requirements . o What makes this organism fastidious? o Is special media needed to grow in culture? If yes, what kind? o Are special environmental conditions needed? If yes, what kind? . Laboratory Identification o Uist the necessary tests and expected results for each needed to fully ientify the organism in the clinical laboratory settingExplanation / Answer
Gardnerella vaginalis
Clinical Significances-
The organism is not always pathogenic, it is anaerobic organism. in anerobic conditions it is established in the vagina. In association with other bacteria Gardnerella vaginalis is reponsible for bacterial vaginosis in women.
It is normal flora found majorily in vaginal fluids and can also detect in blood and urine.
No specific population is vulnerable to this bacteria.
Common infections
Bacterial vaginosis, also found involved in cervical cancer and urinary infection.
Growth Requirements
Colistin and Nalidixic acid make the organism fastidious.
Selective media
Special media is required to grow the bacterium as there is normal microbial flora is also available in the test sample. the other microbe needed to be inhibited so,Gardnerella Selective Agar contains animal peptones, peptic digest of casein, yeast and beef extracts, which provide carbon, nitrogen, amino acids, and essential minerals. Sodium chloride is added to maintain osmotic equilibrium.Colistin, nalidixic acid and amphotericin B are selective agents used to inhibit the growth of other microbes.
As the bacteria is anaerobic so, it needs anaerobic environment to grow. 35- 37 0C temperature.
Laboratory identification
Identification tests
Beta-haemolysis on human blood agar, (susceptibility to trimethoprim and two concentrations of metronidazole, growth in the presence of 2% (w/v) sodium chloride and on nutrient agar, lactic acid production from glucose and P-haemolysis on human-blood agar). ( Refference THE IDENTIFICATION OF GARDNERELLA VAGZNALZS ELIZABETH TAYLOR AND I. PHILLIPS Department of Microbiology, St. Thomas’sHospital Medical School, London SEI 7EH)
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.