5. Using all bioinformatics and computer resources available to you, tell me all

Question

5. Using all bioinformatics and computer resources available to you, tell me all about the following Arabidopsis gene: At3g06440. Specifically, tell me the following information: a. Complete citations of any published scientific papers on this gene and its name; b. Gene sequence and organization (exon/intron); c. The full-length mRNA or cDNA sequence (in FASTA format); d. Protein sequence (in FASTA format); e. Predicted molecular weight of the protein; f. pl; g. Protein targeting sequences and likely cellular destination(s); h. The expression pattern of this gene (microarray data/virtual northern); i. The accession numbers of the three most similar protein sequences related to this translated gene (BLAST)*; j. A sequence alignment of the At3g06440 protein and the three most similar protein sequences*; and k. Design a set of primers to clone this gene into the pRSETB vector that we are using in class. *Note, do not include the same DNA/protein sequence in multiple forms. (30 points, 3 pages' maximum)

Explanation / Answer

a. Complete citation are:

f. Theoretical pI: 7.99

g. Protein is located in cytoplasm

i. Accession no are AXX17_AT3G06200, AAQ77231.1, XP_020887526.1

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