The organisms were E. coli, Streptococcus la tips, and Staphylococcus epidermidi

Question

The organisms were E. coli, Streptococcus la tips, and Staphylococcus epidermidis.

2 In the table below, identity the carbon and nitrogen sourcela) in each medium and then characterize each as "defined" or undefined" (complex"). If undefined, list the ingredientis) that make it undefined. Finally, use your resuilts to characterize each medium based on its ability to support growth of a wide range of organisms (poor, fair, good) Nutrient Broth Brain-Heart Infusion Broth Glucose Salts Broth Medium Description 2 Carbon sourcels) Nitrogen source(s) Defined/undefined Ingredientís) making it "undefined Ability to support a wide range of organisms poor, fair, good) 88 MICROBIOLOGY: Laboratory Theory & Application

Explanation / Answer

Nutrient Broth

Carbon Source: Dextrose is the fermentable carbohydrate providing carbon and energy, beef extract is also the carbon source.

Nitrogen Source: Peptone contribute organic nitrogen in the form of amino acid and long chain fatty acids

Undefined media: because of complex ingredient beef extract

Ability to support wide range of organisms good

Brain Heart Infusion Broth

Carbon Source: Dextrose is the carbohydrate providing carbon and energy, beef extract also serve as the carbon source.

Nitrogen Source: Proteose Peptone contribute organic nitrogen in the form of amino acid and long chain fatty acids

Undefined because of complex ingredient beef extract

Supports growth of the widest range of organism Good

Glucose Salt Broth

Carbon Source: Gucose is the only carbon source in this medium

Nitrogen Source: Hydroxide and ammonium phosphate is the sole source of nitrogen

it is Defined medium

supports growth of fewest organisms Poor

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