) What is a restriction endonuclease? ) In nature, scientists first found endonu
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) What is a restriction endonuclease? ) In nature, scientists first found endonucleases in different bacterial species. What is the function of a restriction endonuclease in bacteria? 4) What is recombinant DNA (rDNA) technology? What is the function of a restriction endonuclease in rDNA technology? ) What is a palindrome? (Look in a dictionary or your English textbook for this one) 6) Give an example of a palindrome 7) What is the SPECIFIC palindromic DNA sequence recognized by Eco RI 8) What is a restriction digest fragment? 9) What is a plasmid? 10) What is a restriction map? 1) How are plasmids used in rDNA technologies? 12) What is the function of DNA ligase in rDNA experimentation? What is the function of DNA ligase during DNA Replication? What do you notice about how ligase works during these two very different processes? 3) Make sure to review the structure of DNA. Campbell text pg. 64-66. Also, look at the lecture PowerPoint for chapter 3 and study the images of DNA. If you aren't already, make sure you are very familiar with this macromolecule. You need to know the overall structure, the overall molecular charge, complementary base pairing rules, orientation of the strands in a DNA molecule, etc 14) What is the overall charge of a DNA molecule? What portion of the molecule gives it this charge?Explanation / Answer
2. Restriction endonuclease is an enzyme used to cut the DNA strand into small fragments.
It is also known as molecular scissor.
3. In bacteria, restriction endonuclease is used to cut the DNA of interest into small fragments.
4. Recombinant DNA technology is the technology in which we join DNA molecules from two different species and insert it into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture and industry.
In rDNA technology, restriction endonuclease is used to cut the DNA of interest.
5. It is a word, phrase, a sequence that reads the same backwards as forwards.
6. Madam.
7. The DNA sequence ACCTAGGT is palindromic because its nucleotide by nucleotide compliment is TGGATCCA.
8. Restriction digest cleaves DNA sequence in a virtual restriction digest, which one, 2 or 3 restriction enzyme.
The resulting fragments fragments are known as restriction digest fragments.
9. It is a genetic structure that can replicate independently of the chromosome.
10. It is a map of known restriction sites within a sequence of DNA.
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