16.On the ribosome, the mRNA is read from A) 5to 3 C- to N-terminus. B) 5 to3:N-

Question

16.On the ribosome, the mRNA is read from A) 5to 3 C- to N-terminus. B) 5 to3:N-to C-terminus. C) 3 to 5: C-to N-terminus D) 3 tO 5: N-to C-terminus. and the polypeptde chain is synthesized from 17. An elongating ribosome is bound to appropriate IRNAs in both the A and the P sites and is ready for peptidyl transfer. What happens next? A) The carboxyl end of the polypeptide chain is released from the P-site tRNA and joined to the free amino group of the amino acid linked to the A-site RNA B) The amino end of the polypeptide chain is released from the P-site tRNA and joined to the free carboxyl group of the amino acid linked to the A-site RNA The carboxyl end of the amino acid is released from the A-site tRNA and joined to the free am ino group of the polypeptide chain linked to the P-site RNA The amino end of the amino acid is released from the A-site tRNA and joined to the free carboxyl group of the polypeptide chain linked to the P site tRNA C) D) 18. In the following schematic graph of a hypothetical set of RNA-seq data, the number of reads is plotted for a region of chrom osome containing two genes, from samples obtained from two diferent tissues. Which gene (X or Y) do you think is more hikely a housekeeping" gene? Which region (1 or 2) within gene Y most likely corresponds to an exon? Liver Brain Gene X Gene ? Position on chromosome A) Gene X; region B) Gene x; region 2 C) Both genes; region 1 D) Gene Y: region E) Gene Y, region 2 19. Studying the expression of a transcription regulatory protein in two cell types, you have performed experiments showing that the mRNA encoding the protein is present at comparable levels in the Cytosol of both cell types. However, based on the expression of its target genes, you suspect that the protein activity might be significantly different in the two cell types. Which of the following steps in expression of the gene encoding this protein is more likely to be dfferentially controlled in these cel types? A) Transcription B) Translation C) mRNA transport D) mRNA degradation

Explanation / Answer

Ans 16:

During protein synthesis mRNA brings codons which is read in the 5'-3' direction by the ribosome. The polypeptide is synthesised in the direction N terminal (amino, NH2) to C terminal (carboxy, COOH).

Thus corret choice should be

(b) 5'-3' and N to C terminus.

Ans 17

(A) the carboxyl end of tRNA at p site is joined to amino group of tRNA at A site.

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