.Explain the following in detail on the basis of staining techniques, 1.For eith

Question

.Explain the following in detail on the basis of staining techniques,

1.For either plate # 1 (floor sample )or plate # 2 (arm sample ), discuss whether or not all the organisms on your swab

resulted in colony growth on TSA media incubated at 35 C for 48 hours?

2.Explain why TSA media is not designated as either

selective or differential.

3.Explain why methylene blue stain cannot be used for negative stain techniques.

4.State three reasons why it is necessary to heat fix the smear prior to applying a

positive stain.

Explanation / Answer

4.Ans-
Heat fixation is an important step for preparing slides and samples in microbiology, pathology, and histology. The reasons for the importance of heat fixing include:
A. The heat fixing a smear helps stick the cells to slide and prevent damage over the time.
B. It prevents any type of biological or chemical reactions that take place on the slide.
C. It helps to preserve cells and tissue samples for further study.

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