Explain how the E. coli at the beginning of Dr. Lenski\'s long-term evolution ex

Question

Explain how the E. coli at the beginning of Dr. Lenski's long-term evolution experiment used the growth medium as a food source to obtain energy. Explain the novel way the E. coli in the Ara-3 flask (#9) of Dr. Lenski's long-term evolution experiment are using the growth medium* as a food source to obtain energy. Discuss only the differences between the bacteria in this flask versus all other flasks. Do not describe the mutational changes that led to this evolutionary novelty, but do discuss the effects of these mutations (i.e. explain the phenotypes).

Explanation / Answer

Ans 1) The experiment was carried out by Lenski with the bacteria that were grown in a glucose limited minimal medium called as DM25. It was used as food source to obtain energy as it was used for isolating auxotrophic mutants of E.coli in presence of penicillin as selective agent. DM25 along with low concentration of glucose was supplemented and the DM24 consists of large amount of citrate. It was used initially to improve the killing efficiency during the experiments.

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