11. What are Okazaki fragments? (2 pt) a. short DNA pieces that explain how DNA
ID: 273522 • Letter: 1
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11. What are Okazaki fragments? (2 pt) a. short DNA pieces that explain how DNA is synthesized on the lagging strand b. short DNA pieces that explain how DNA is synthesized on the leading strand. c. the remnants of the original strands that are dispersed in the new double stranded DNA molecules d. RNA primers used for DNA replication. e. None of the above 12. How do the core enzyme and the holoenzyme of RNA polymerase differ in E. coli? (2 pt) a. The holoenzyme includes the sigma (o) subunit, the core enzyme does not. b. The core enzyme includes the sigma (o) subunit, the holoenzyme does not c. The holoenzyme transerbes from an RNA template, the core enzyme from a DNA template. d. The core enzyme transcribes from an RNA template, the holoenzyme from a DNA template. e. None of the above 13. Which below is not a DNA strand used to direct RNA synthesis? (2 p) a. Template strand b. Coding strand c. Antisense strand d. Negative or" strand e. None of the above enzyme? (2 pt) 14. At what point does the sigma (?) subunit of RNA polymerase released from the core a. Prior to the incorporation of any nucleotides into an RNA strand. b. After transcription begins and about 10 nucleotides have been added to the RNA chain. c. Just prior to chain termination. d. Never; it is an intrinsic part of the core enzyme e. After RNA polymerase discovers an inverted repeat 15. What is the function of the sigma (o) subunit of RNA polymerase in E. coli? (2 pt) a. It recognizes promoters where transcription should begin. b. It contains the active site for synthesis of RNA c. It ensures proper processitvity of the polymerase, so it doesn't stop prematurely. d. It is involved in chain termination. e. It scrunches the DNAExplanation / Answer
11. a
Okazaki fragments are short pieces of DNA that are repeatedly synthesized on the lagging-strand template.
12. a
Holoenzyme can be separated into two components, the core enzyme and the sigma factor.
13. b
RNA synthesis occurs from the template or antisense or negative strand (-). The RNA transcribed has the same sequence as that of coding strand or sense strand or positive (+) strand (except for U instead of T).
14. b
The sigma facror dissociates from the promoter after about 10 nucleotides have been added. This is called as promoter clearance. It marks the beginning of elongation phase.
15. a
Sigma factor confers the ability to recognize consensus sequence within the promoter.
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