1. Amino acids 51-56 were compared for an E coli metabolic enzyme from a wild ty
ID: 273064 • Letter: 1
Question
1. Amino acids 51-56 were compared for an E coli metabolic enzyme from a wild type strain and a strain with a double frameshift mutation, having one nucleotide deletion followed by one nucleotide insertion at another position. The results are shown below. Amino Acid Residue 51 52 53 54 55 56 Wild Type NH ALA ASP PRO GLN VAL ILECOOH Double Mutant NHr | GLU | THR | LEU | LYS I TRP | ILE 1 -COOH a. Determine the base sequence of the mRNA for both the wild type and b. Using your wild type mRNA sequence, show which base was deleted and c. Using your mutant sequence, show which base was added and where it double mutant polypeptides. Be sure to show which bases are certain and which have uncertainty, if any. the site from which it was removed. Be sure to show which bases are certain and which have uncertainty, if any was inserted. (You only need to give one solution if there are many.) UUU- phenylalanine PHE UCU serine SER UCC = serine UUC = phenylalanine UUA = leucine UUG leucine cuU leucine CUC = leucine CUA leucine CUG leucine AUU isoleucine AUC isoleucine AUA, isoleucine LEU UCA serine UCG serine CCU-proline PRO CCC proline CCA-proline CCG = proline ILE ACU= threonine THR ACC threonine ACA threonine AUG methionine (start) MET ACG-threonine GUU valine GUC valine GUA valine GUG valine VAL GCU = alanine ALA GCC alanine GCA alanine GCG-alanine UAU tyrosine YR UAC tyrosine UAA-stop UAG-stop CAU = histidine CAC histidine UGU -cysteine UGC cysteine UGA = st CYS UGG tryptophan TRP CGU- arginine ARG CGC = arginine CGA arginine CGG = arginine AGU serine AGC serine HIS CAA glutamine GLN CAG glutamine AAU- asparagine ASN AAC-asparagine AAA-lysine. LYS AAG-lysine GAU = aspartic acid ASP GAC = aspartic acid GAA-glutamic acid GLU GAG glutamic acid 1 AGA-arginine IGGU-glycine SER ARG GLY AGG-arginine GGC glycine GGA-glycine GGG glycineExplanation / Answer
a) mRNA sequences: certain bases have been shown in bold; while uncertain bases, if more than one base is possible at that particular position, have been shown, within parentheses() and "/" symbol for alternative bases. Each triplet codon has been separated using "|" symbol. In wild type the postion of deleted nucleotide has been shown using inverted comma ('C') and the bas is written in italics. Also for mutant sequence insertion has been show same way i.e keeping the inserted nucleotide inside inverted comma ('G') and written in italics.
i) Wild type sequence: G'C'A|GAC|CCU|CAA|GUG|AU(U/C)
ii) Mutant sequence: GAG|ACC|CUC|AAG|UG'G'|AU(U/C)
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