Name Recitation head (34 pts) In a newly discovered animal, chromosome 3 contain
ID: 272515 • Letter: N
Question
Name Recitation head (34 pts) In a newly discovered animal, chromosome 3 contains a dominant mutation stripe, which causes a recessive lethal phenotype during larval stages. Several mutant animals carrying recessive lethal mutations on chromosome 3 were isolated an (i.e., mutant/stripe). These stocks were then crossed to each other (i.e., mutl/stripe x mut2/stripe) and their progeny (including dead carcasses) were examined. Listed in the table below is the number of progeny that are wild type (i.e., NOT stripe). d kept in stocks as trans-heterozygotes with stripe mutant # 250 0 221 250 0 300 10 0 280 120 140 0 150 180120 130 180 0 200 160 225 130 210 360 0 158 0 100 175 201 101 130 150 170 0 350 280 260 330 256 0 180 280 10 3501 165 | 190 | 220 | 145 | 310 ? 1250 -1195-10 A. What are the complementation groups? B. You isolate 9 additional non-lethal mutants that are in the same complementation group as mutant 3. EcoR orimer 2 primer 1 The sequence below is the region surrounding gene X. Design DNA primers 1 and 2 (5 nucleotides ONLY) to amplify this region. Indicate the polarity of the primers. Primer 2: 5'- I. Primer 1: 5'- II. Which is the template strand for transcription? Circle ONE: A or B III. Which is the 3' end of the DNA? Circle ONE: A or BExplanation / Answer
6 Complementation groups
complementation groups-- (1,5), (2,4), (3), (6,9), (7,10), (8)
B.
Strand A- 5’-3’
Strand B- 3’-5’
I. Primer1: 5’-AGCAT-3’
Primer2: 5’-CTCGC-3’
II. Template strand for transcription- strand B
III. 3’ end- B
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