D. (4pts) Lastly, you analyze a mutant cell line. In this cell line, a mutant fo
ID: 272036 • Letter: D
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D. (4pts) Lastly, you analyze a mutant cell line. In this cell line, a mutant form of the protein YFG is always active, and that causes the cells to progress through G1 more rapidly. You perform the above experiment. Please draw what you predict will be the resulting histogram after you measure BrdU in the figure to the right. (Note: The result from the control is printed as a reference, you should draw your response on the same axes.) E. (2pts) Would you characterize the YFG gene as an oncogene or a tumor suppressor? 0 1 2 Fluorescence intensity relative units) 4. You and your postdoctoral mentor are testing two newly developed anti-cancer drugs. You have obtained two sets of patient samples to test the drugs on: cancer cells and normal cells. You go into lab to finish an experiment for your postdoc while they are at a conterence. Your postdoc's handwriting is terrible, so you aren't sure which plate has the cancer cells and which has the normal cells based on their labeling. You do know that one plate was seeded with cancer cells, and one plate was seeded with normal cells. You also know that you and the postdoc included 2 wells treated with NO drug, 2 wells treated with Drug A, and two wells treated with Drug B You decide to finish the experiment anyway and check in with your postdoc when they return. You stain the cells with crystal violet to identify the level of foci formation on all plates. Below are photos of the results you obtained. Cell samples: 1)Pancreatic cancer cells from a patient tumor OR 2)Normal cells from normal patient tissue Treatments: No drug, Drug A or Drug B (All treatments were completed in duplicate.) Results PLATE 1 PLATE 2 2 2 2 3Explanation / Answer
Answer D and E. For these question, it needs more information. It will be helpful if you post the whole question.
Answer 4. A. No, both the plates were not seeded with normal cells. As it is already mentioned in the question that one plate is seeded with normal cells and one with pancreatic cancer cells. Also, Plate no.1 seems to be pancreatic cancer cell seeded plate as the number of cells is more in plate 1, and uncontrollable and fast division of cells is the basic property of cancer cells.
B. Both the wells named 1 in Plate 1 and Plate 2 (normal cells won't grow as fast as cancer cells) most likely seem to be control cells as they have grown substantially as can be seen by the crystal violet staining.
C. The wells labelled as 2 and 3 are treated with Drug. However it is impossible to determine which well was treated with Drug A and which was treated with Drug B as both the drugs seem to inhibit cellular proliferating in a very similar manner.
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