Temperature sensitive (ts) mutations of phage T4 grows only at 30°C whereas the

Question

Temperature sensitive (ts) mutations of phage T4 grows only at 30°C whereas the wild type (ts*) phage T4 can grow at both 30°C and 40°C. You want to determine the recombination frequency between two ts mutations (called ts1 and ts2). An E. coli strain is coinfected (at high m.o.i.) at 30°C with a wild type phage stock and with a phage that contains both ts mutations. ts1 ts2 x ts1+ ts2 When the progeny are titered it is determined that 1 x 109 pfu/ml of the progeny phage stock can form plaques at 30°C but only 3.5 x 108 pfu/ml can form plaques at 40°C. What is the recombination frequency between the two ts mutations? (circle correct answer) A. 10% B. 15% C. 20% D. 30% E. 35%

Explanation / Answer

It was known that the temperate sensitive mutation phages grow at 30o C and wild type phages grows in both 30C and 40o C. The recombination between this two i.e, both wild type and mutant strain, most of the progeny formed plaques at 40o C and remaining proge y formed at 30o C. So it was identified that about 30% of recombinant frequancy is identified in ts mutations.

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